CD32 expression is associated to T-cell activation and is not a marker of the HIV-1 reservoir

CD32 expression is associated to T-cell activation and is not a marker of the HIV-1 reservoir

Online publication: 16/07/2018 Journal: Nature Communications

Abstract:

CD32 has been shown to be preferentially expressed in latently HIV-1-infected cells in an in vitro model of quiescent CD4 T cells. Here we show that stimulation of CD4+ T cells with IL-2, IL-7, PHA, and anti-CD3/CD28 antibodies induces T-cell proliferation, co-expression of CD32 and the activation of the markers HLA-DR and CD69. HIV-1 infection increases CD32 expression. 79.2% of the CD32+/CD4+ T cells from HIV+ individuals under antiretroviral treatment were HLA-DR+. Resting CD4+ T cells infected in vitro generally results in higher integration of provirus. We observe no difference in provirus integration or replication-competent inducible latent HIV-1 in CD32+ or CD32− CD4+ T cells from HIV+ individuals. Our results demonstrate that CD32 expression is a marker of CD4+ T cell activation in HIV+ individuals and raises questions regarding the immune resting status of CD32+ cells harboring HIV-1 proviruses.

Authors: Roger Badia, Ester Ballana, Marc Castellví, Edurne García-Vidal, Maria Pujantell, Bonaventura Clotet, Julia G. Prado, Jordi Puig, Miguel A. Martínez, Eva Riveira-Muñoz, José A. Esté. 

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