Evidence for preferential genotyping of a minority human immunodeficiency virus population due to primer-template mismatching during PCR-based amplification

Evidence for preferential genotyping of a minority human immunodeficiency virus population due to primer-template mismatching during PCR-based amplification

Data de publicació online: 01/01/2005 Revista: Journal of Clinical Microbiology

Abstract:

Human immunodeficiency virus type 1 (HIV-1) genotyping assays have come to be widely used for monitoring antiretroviral drug resistance. We report a case in which primer-template mismatches during nested PCR-based amplification biased the composition of the original viral population in the sample, magnifying a distinct minority HIV-1 population. This observation might help to explain some unexpected HIV-1 genotypes.

Autors: Javier Martinez-Picado, Kristina Morales-Lopetegi, Cristina Villena, Carolina Gutiérrez, Nuria Izquierdo, Silvia Marfil, Bonaventura Clotet, and Lidia Ruiz.

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